Effects of different amounts of cellulase on the microstructure and soluble substances of cotton stalk bark

Abstract There are plant cuticle and complex cell walls on the outer surface of cotton stalk bark (CSB), which reduce efficiency liquid penetration CSB. To increase permeability liquids, these barriers need to be broken. Cellulase can selectively hydrolyze cellulose into glucose, its action conditions mild. Therefore, cellulase treatment is one excellent ways break CSB permeation barrier. This experiment studied effects different amounts enzymatic hydrolysis products Scanning electron microscopy (SEM) nano-CT were used observe changes in microscopic morphology Ion chromatography an ultraviolet–visible spectrophotometer determine dissolution The results showed that treated with was broken, wall phloem fibers became thinner, increased accessibility liquid. content monosaccharide lignin solution dosage. Correspondingly, proportion polysaccharides continued decline eventually stabilized. These experimental provide a reference for improving natural subsequent biomass products. Graphical abstract pretreatment barks destroyed dense protective structure wall,